Morrisville, North Carolina
Framingham, Massachusetts
Maryland
Boston, Massachusetts
Boston, Massachusetts
Cincinnati, Ohio
Berkeley, California
Durham, North Carolina
Posted: 14-Mar-23
Location: La Jolla, California
Salary: Open
Internal Number: 122017
UCSD Layoff from Career Appointment: Apply by 03/13/2023 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 03/23/2023. Eligible Special Selection clients should contact their Disability Counselor for assistance.
DESCRIPTION
UCSD Health Sciences is a $1 billion dollar plus organization encompassing 16 academic departments (14 clinical, 2 basic sciences), 2 pre-academic/hospital departments, 2 professional schools (School of Medicine and SkaggsSchool of Pharmacy and Pharmaceutical Sciences), 2 hospitals, and various other programs and units all dedicated towards fulfilling the Health Sciences missions. The UCSD Department of Cellular and Molecular Medicine (CMM) is one of two basic science units in the School of Medicine where world-class researchers study fundamental cellular processes and pathways using a variety of techniques from classical genetics and fluorescence microscopy to high-throughput genomics, systems biology, and crystallography.
Our 36 faculty and over 200 postdoctoral scholars, graduate students, and research staff work on the cutting edge of biology, cutting across fields from basic biochemistry and genetics to genomics, systems biology and stem cell biology. The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines and provide diversity to meet the interests of a broad spectrum of students and scholars.
The diverse mix of ages, backgrounds, and talents creates a robust work environment withchallenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced, and deeply interested individuals who are ready to become a part of our dynamic research and educational environment.
Under supervision the Research Associate will assist with and perform tasks related to cell culture, passaging and transfections; animal (mice) husbandry, euthanasia and dissection; cell analysis by flow cytometry and microscopy; biochemistry and molecular biology techniques such as DNA and RNA extractions; quantitative PCR; protein extraction and purification, immunoblotting as well as performing general laboratory support such as ordering of laboratory supplies, stocking solutions and attending regular laboratory meetings.
MINIMUM QUALIFICATIONS
Theoretical and practical knowledge of cellular and molecular biology and tissue culture or related field or combination of education and experience.
Knowledge of Molecular Biology techniques.
Experience propagating tissue culture cells including hESC and hiPSC lines.
Experience working with tissue culture cells under sterile conditions. Demonstrated working experience with plasmids, sterile technique, preparation of solutions/media, bacteriology & molecular cloning.
Capability performing DNA/RNA extractions, purification and manipulation.
Demonstrated knowledge of experimental work or general laboratory experience.
Ability to calculate and prepare chemical solutions and learn new procedures.
Proven ability to follow experimental protocols and skills to develop accurate data from results.
Ability to work with a variety of laboratory equipment, including but not limited to PCR machines, luminometers, incubators, centrifuges, autoclaves, spectrophotometers, & darkroom equipment.
Strong verbal and written communication skills to be effective in communicating & interacting with a diverse group of people within a laboratory setting.
Demonstrated computer skills, & experience with various software packages. Demonstrated ability to collect, reduce and analyze data.
PREFERRED QUALIFICATIONS
- Experience with flow cytometry to analyze cell populations.
- Experience laboratory safety including radioactive/biohazard handling & disposal techniques, and ability to ensure laboratory compliance with required safety.
- Ability to complete protein extractions and immunoblotting and experience with protein purification using column chromatography.
- Experience with animal (mice) husbandry.
- Experience producing retro/lenti viral supernatants and infecting mammalian cell lines.
SPECIAL CONDITIONS
- Job offer is contingent upon satisfactory clearance based on Background Check results.
- Occasional evenings and weekends may be required.
- Must be willing to work with human embryonic stem cells and animals (mice).
- Must be willing to work with infectious agents, e.g. lenti viruses.
- Must n Connections working at University of California San DiegoUC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.
