Junior/Assistant/Associate/Full Specialist (Protein Engineering)
Antibiome Center, Department of Pharmaceutical Chemistry UCSF

The laboratory of Jim Wells and the Antibiome Center is seeking a highly motivated candidate to join our quick paced and multi-talented team. The successful candidate will be responsible for molecular biology, antibody display library, protein/antibody expression and purification, and protein/antibody validation. The candidate will have opportunities to learn state-of-the-art techniques. Our laboratory is highly collaborative and is engaged in numerous academic and industrial collaborative projects.

The Antibiome Center is part of the Recombinant Antibody Network (RAN), a collaborative consortium consisting of laboratories at the University of California San Francisco (Dr. Jim Wells), University of Chicago (Dr. Tony Kossiakoff), and the University of Toronto (Dr. Sachdev Sidhu). We are committed to generate recombinant antibodies at the surface proteome-wide scale using high throughput phage display approaches. Our long history of protein engineering expertise also enables the development of bio-therapeutic antibodies in the immuno-oncology field.

Primary responsibilities

• Cloning recombinant protein and antibody construct using a variety of molecular biology techniques including Polymerase Chain Reaction, Gibson assembly, DNA purification, and transformation.
• Antibody display library generation (phage, yeast, or mammalian).
• Protein expression from bacterial and mammalian sources.
• Purification of proteins using chromatography.
• Characterization of proteins by variety of techniques including SDS-PAGE, ELISA, SEC, and Western Blotting.

Required Qualifications

• Appointees to the Junior rank must possess a bachelor's degree (or equivalent) in biochemistry, cell biology, or relevant life sciences field or at least four years of relevant research experience.
• Appointees to the Assistant/Associate rank should possess a master's degree (or equivalent) in a relevant life sciences field or at least five years to ten years of research experience.
• Appointees to the Full rank must possess a PhD (or equivalent) in relevant life sciences field or at least ten or more years of research experience.

Preferred Qualifications

• One year of wet lab experience
• Expertise with display library generation and selection.
• Experience with Next-Generation-Sequencing techniques and analysis.
• Familiar with mammalian tissue culture techniques including transfections, cell line maintenance, recombinant protein expression.
• Proficiency with advance biophysical and structure characterization of proteins and antibodies.
• Data analysis experience using programing languages such as R or Python.

Please apply online at https://aprecruit.ucsf.edu/JPF04105 with a cover letter, CV, and the contact information of three references. Applicant materials must list current and/or pending qualifications upon submission. The selected candidate must meet all of the qualifications at the time of appointment.

See Table 24B (https://www.ucop.edu/academic-personnel-programs/_files/2022-23/july-2022-salary-scales/t24-b.pdf) for the salary range for this position. A reasonable estimate for this position is $49,000-$173,900.

As a condition of employment, you will be required to comply with the University of California SARS-CoV-2 (COVID-19) Vaccination Program Policy found here: https://policy.ucop.edu/doc/5000695/SARS-CoV-2_Covid-19.

UC San Francisco seeks candidates whose experience, teaching, research, or community service that has prepared them to contribute to our commitment to diversity and excellence. The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status.