We are looking for a research assistant II excited about translational research to join professor Robert Langer and Daniel Anderson's laboratory at the Koch Institute of Integrative Cancer Research, MIT to support research projects on developing peptide-based therapeutics for treating diabetes. This project is funded by a grant through Boston Children's Hospital. This is a highly interdisciplinary project at the intersection of bioconjugation chemistry, molecular biology, protein engineering and bioengineering; and involves multiple collaborations. The role will involve running in vitro/in vivo assays for evaluating hydrogels for drug release, in vitro/in vivo assays for evaluating modified peptides, various in vitro and cell based binding assays. In addition, the person will be expected to maintain accurate laboratory records, and stock laboratory supplies. This is a great opportunity for engineers interested in gaining research experience in an interdisciplinary area combining engineering with translational research before applying for graduate/medical school.
Principal Duties and Responsibilities
Performs in vitro testing of drugs for release from the devices using a variety of laboratory protocols and equipment. Records results accurately and troubleshoots procedures.
Maintain and perform biomolecular assays (in vitro radioligand binding assays, ELISA, SDS PAGE, western blots, and others).
Performs analysis using mass spectrometric data for quantification of various peptides from plasma samples.
Performs cell based binding assays.
Keeps accurate logs of all experiments performed and communicates findings to supervisor.
A ttends courses in safety procedures for handling, storage and disposal of biohazardous, flammable, corrosive and toxic materials. Carries out all laboratory activities in compliance with applicable regulations and protocols.
Conducts literature searches and reviews, compiling information for journals and suggesting uses for new techniques to the P.I. Attends courses, lectures and conferences to further own knowledge of research and laboratory techniques, consulting with other researchers to exchange information.
Contributes to the teamwork within and between departments and organizations.
Minimum Knowledge and Skills required by the Job
The knowledge of theories, principles, and concepts acquired through completion of a BS/MS degree in chemistry/ Molecular biology/ biochemistry/ pharmacy/ pharmaceutical chemistry/ pharmacology or any closely related field, and previous lab experience.
Staff will be trained to use the relevant techniques in NMR, mass spectrometry but previous experience with some of the following techniques would be advantageous;
In vitro and cell based binding assays (radioligand based, other biophysical techniques)
ELISA, SDS PAGE, Western Blot, plasma sample analysis
The analytical skills to resolve problems that require the use of basic scientific, mathematical, or technical principles. Work requires the ability to exchange information on factual matters, schedule appointments, keep accurate records of procedures in the lab and good communication skills with other team members.
Blood Borne Pathogen Category
Potential Exposure. Job requires performance of duties that involve potential for exposure to blood, body fluids, or tissues.
Boston Children's Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.
Internal Number: 3013808
About Boston Children's Hospital
Boston Children's Hospital is a 395-bed comprehensive center for pediatric health care. As one of the largest pediatric medical centers in the United States, Children's offers a complete range of health care services for children from birth through 21 years of age.